技术文章
测量应用案例-20220603
阅读:194 发布时间:2022-6-14
文献名:
Similar toxicity mechanisms between graphene oxide and oxidized multi-walled carbon nanotubes in Microcystis aeruginosa
作者
:
Edgardo Cruces
a
,
Ana C. Barrios
bc
,
Yaritza P. Cahue
bc
,
Brielle Januszewski
bc
,
Leanne M. Gilbertson
de
,
François Perreault
bc
a
Centro de Investigaciones Costeras Universidad de Atacama, Avenida Copayapu 485, Copiapo, Chile
b
School of Sustainable Engineering and the Built Environment, Arizona State University, Tempe, AZ, 85287-3005, USA
c
Nanosystems Engineering Research Center for Nanotechnology-Enabled Water Treatment, United States
d
Department of Civil and Environmental Engineering, University of Pittsburgh, Pittsburgh, PA, 15261, USA
e
Department of Chemical and Petroleum Engineering, University of Pittsburgh, Pittsburgh, PA, 15261, USA
摘要:
In photosynthetic
microorganisms
, the toxicity of carbon
nanomaterials
(CNMs) is typically characterized by a decrease in growth, viability, photosynthesis, as well as the induction of
oxidative stress
. However, it is currently unclear how the shape of the carbon structure in CNMs, such as in the 1-dimensional
carbon nanotubes
(CNTs) compared to the two-dimensional graphene oxide (GO), affects the way they interact with cells. In this study, the effects of GO and oxidized multi-walled CNTs were compared in the cyanobacterium
Microcystis
aeruginosa
to determine the similarities or differences in how the two CNMs interact with and induce toxicity to cyanobacteria. Using change in Chlorophyll
a
concentrations, the effective concentrations inducing 50% inhibition (EC
50
) at 96 h are found to be 11.1 μg/mL and 7.38 μg/mL for GO and CNTs, respectively. The EC
50
of the two CNMs were not found to be statistically different. Changes in fluorescein diacetate and 2′,7′-dichlorodihydrofluorescein diacetate fluorescence, measured at the EC
50
concentrations, suggest a decrease in esterase
enzyme activity
but no oxidative stress. Scanning and
transmission electron microscopy
imaging did not show extensive membrane damage in cells exposed to GO or CNTs. Altogether, the decrease in metabolic activity and photosynthetic activity without oxidative stress or membrane damage support the hypothesis that both GO and CNTs induced indirect toxicity through physical mechanisms associated with light shading and cell aggregation. This indirect toxicity explains why the intrinsic differences in shape, size, and surface properties between CNTs and GO did not result in differences in how they induce toxicity to cyanobacteria.
关键词:
Graphene oxide
;
Carbon nanotubes
;
Toxicity
;
Microcystis aeruginosa
;
Photosynthesis
;
Oxidative stress