Spatiotemporal Distribution of Phenolamides and the Genetics of Natural Variation of Hydroxycinnamoy-资料下载-上海安谱实验科技股份有限公司

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Spatiotemporal Distribution of Phenolamides and the Genetics of Natural Variation of Hydroxycinnamoy

阅读：512 发布时间：2018-01-16

Phenolamides constitute a diverse class of secondary metabolites that are found ubiquitously in plants and have been implicated to play an important role in a wide range of biological processes, such as plant development and defense. However, spatiotemporal accumulation patterns of phenolamides in rice, one of the most important crops, are not available, and no gene responsible for phenolamide biosynthesis has been identified in this species. In this study, we report the comprehensive metabolic profiling and natural variation analysis of phenolamides in a collection of rice germplasm using a liquid chromatography–mass spectrometry-based targeted metabolomics method. Spatiotemporal controlled accumulations were observed for most phenolamides, together with their differential accumulations between the two major subspecies of rice. Further metabolic genome-wide association study (mGWAS) in rice leaf and in vivo metabolic analysis of the transgenic plants identified Os12g27220 and Os12g27254 as two spermidine hydroxycinnamoyl transferases that might underlie the natural variation of levels of spermidine conjugates in rice. Our work demonstrates that gene-to-metabolite analysis by mGWAS provides a useful tool for functional gene identification and omics-based crop genetic improvement在这项研究中,我们报告使用基于LC-MS的靶向代谢组学方法在一组水稻种质中对酚酰胺的全面代谢谱和自然变异分析。大多数酚酰胺的时空控制积累,以及两大亚种之间的差异积累。进一步的水稻代谢全基因组关联研究（mGWAS）和转基因植物的体内代谢分析将Os12g27220和Os12g27254鉴定为两种亚精胺羟基肉桂酰基转移酶,其可能是水稻中亚精胺结合物水平的自然变异的基础。我们的工作表明,由mGWAS进行的基因到代谢物分析为功能基因鉴定和基于组学的作物遗传改良提供了有用的工具。

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Spatiotemporal Distribution of Phenolamides and the Genetics of Natural Variation of Hydroxycinnamoy

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